Generator

Part:BBa_K524003:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST   (2011-09-28)

nadE gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nadE gene in the E. coli genome is originally terminated by downstream extragenic sites. The extragenic sties were replaced with the double terminator BBa_B0015 in our construct.

Source

The nadE gene was cloned out from the genome of E. coli strain BL21(DE3).

References

Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism